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ID 51875
フルテキストURL
著者
Kikuchi, Takeshi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
Kubota, Satoshi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
Asaumi, Koji Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Kawaki, Harumi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
Nishida, Takashi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
Kawata, Kazumi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
Mitani, Shigeru Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Tabata, Yasuhiko Kyoto Univ, Inst Frontier Med Sci, Dept Biomat
Ozaki, Toshifumi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Takigawa, Masaharu Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent
抄録
CCN family protein 2/connective tissue growth factor (CCN2/CTGF) is a unique molecule that promotes the entire endochondral ossification process and regeneration of damaged articular cartilage. Also, CCN2 has been shown to enhance the adhesion and migration of bone marrow stromal cells as well as the growth and differentiation of osteoblasts; hence, its utility in bone regeneration has been suggested. Here, we evaluated the effect of CCN2 on the regeneration of an intractable bone defect in a rat model. First, we prepared two recombinant CCN2s of different origins, and the one showing the stronger effect on osteoblasts in vitro was selected for further evaluation, based on the result of an in vitro bioassay. Next, to obtain a sustained effect, the recombinant CCN2 was incorporated into gelatin hydrogel that enabled the gradual release of the factor. Evaluation in vivo indicated that CCN2 continued to be released at least for up to 14 days after its incorporation. Application of the gelatin hydrogel-CCN2 complex, together with a collagen scaffold to the bone defect prepared in a rat femur resulted in remarkable induction of osteoblastic mineralization markers within 2 weeks. Finally, distinct enhancement of bone regeneration was observed 3 weeks after the application of the complex. These results confirm the utility of CCN2 in the regeneration of intractable bone defects in vivo when the factor is incorporated into gelatin hydrogel.
発行日
2008-06
出版物タイトル
Tissue Engineering Part A
14巻
6号
出版者
Mary Ann Liebert, Inc.
開始ページ
1089
終了ページ
1098
ISSN
1937-3341
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1089/ten.tea.2007.0167
言語
English
OAI-PMH Set
岡山大学
著作権者
© Mary Ann Liebert, Inc.
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT