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ID 13572
Eprint ID
13572
フルテキストURL
著者
伊東 秀之 岡山大学薬学部 Kaken ID publons
Marie-Paule Gonthier Unité des Maladies Métaboliques et Micronutrients
Claudine Manach Unité des Maladies Métaboliques et Micronutrients
Christine Morand Unité des Maladies Métaboliques et Micronutrients
Louise Mennen UMR INSERM U557/INRA/CNAM
Christian Rémésy Unité des Maladies Métaboliques et Micronutrients
Augustin Scalbert Unité des Maladies Métaboliques et Micronutrients
抄録
Dietary polyphenols are suggested to participate in the prevention of CVD and cancer. It is essential for epidemiological studies to be able to compare intake of the main dietary polyphenols in populations. The present paper describes a fast method suitable for the analysis of polyphenols in urine, selected as potential biomarkers of intake. This method is applied to the estimation of polyphenol recovery after ingestion of six different polyphenol-rich beverages. Fifteen polyphenols including mammalian lignans (enterodiol and enterolactone), several phenolic acids (chlorogenic, caffeic, m-coumaric, gallic, and 4-Omethylgallic acids), phloretin and various flavonoids (catechin, epicatechin, quercetin, isorhamnetin, kaempferol, hesperetin, and naringenin) were simultaneously quantified in human urine by HPLC coupled with electrospray ionisation mass-MS (HPLC-electrospray-tandem mass spectrometry) with a run time of 6 min per sample. The method has been validated with regard to linearity, precision, and accuracy in intra- and inter-day assays. It was applied to urine samples collected from nine volunteers in the 24 h following consumption of either green tea, a grape-skin extract, cocoa beverage, coffee, grapefruit juice or orange juice. Levels of urinary excretion suggest that chlorogenic acid, gallic acid, epicatechin, naringenin or hesperetin could be used as specific biomarkers to evaluate the consumption of coffee, wine, tea or cocoa, and citrus juices respectively.
キーワード
Polyphenols
Flavonoids
Phenolic acids
High-throughput analysis
Urine
Beverages
発行日
2005-10
出版物タイトル
British Journal of Nutrition
94巻
4号
出版者
The Nutrition Society
開始ページ
500
終了ページ
509
ISSN
0007-1145
NCID
AA00574708
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1079/BJN20051522
言語
English
著作権者
The Nutrition Society
論文のバージョン
publisher
査読
有り
DOI